Results for 6bgi

PDB: 6bgi Gene name: Ano1 UniProt: ANO1_MOUSE EMD: emd_7095.gz

Membrane mimetics: nanodisc;

Cross-sections at different angles

green: density, gray dots: atoms from the all-atom model, lines: membrane boundaries defined by TMDET

The determined edges at each slice and their comparison to TMDET

dots: edges of the gradient, triangles: outliers,
dashed and solid lines: membrane boundaries defined by TMDET and MemBlob ( mean(Zi) decreased by the thickness of the interface region (8 Å))
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Manual settings applied

140-190 end1 around 25 3
320-350 end1 around 20 3
100 end0 average 90 110
110 end1 average 100 120
280 end0 average 270 290
0 end1 equal 10
280 end1 equal 270
290 end1 equal 300

Boundary values (averages for MemBlob)

Z (PDBTM) Z (MemBlob) Z - 8
End1 13.75 20.22 12.22
End0 -13.75 -20.78 -12.78

Mapping the results to the structure

You can move/scale/rotate the structure. Blue: a.a. facing to bulk water; magenta: interface region; green: a.a. interacting with the hydrophobic membrane core; yellow: burried a.a. red: a.a. with an invisible side chain and non-protein molecules, for which DSSP cannot return SASA
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