Results for 5w3s

PDB: 5w3s Gene name: MCOLN3 UniProt: MCLN3_CALJA EMD: emd_8764.gz
EMD-8764
Membrane mimetics: amphipol; Note: More manual adjustment needed because of the noise level

Cross-sections at different angles

green: density, gray dots: atoms from the all-atom model, lines: membrane boundaries defined by TMDET



The determined edges at each slice and their comparison to TMDET

dots: edges of the gradient, triangles: outliers,
dashed and solid lines: membrane boundaries defined by TMDET and MemBlob (mean(Zi) decreased by the thickness of the interface region (8 Å))
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Manual settings applied

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Boundary values (averages for MemBlob)


Z (PDBTM) Z (MemBlob) Z - 8
End1 17.50 22.00 14.00
End0 -17.50 -27.50 -19.50